Data from: Changes in the chlorophyll content of grape leaves could provide a physiological index for responses and adaptation to UV-C radiation

Stilbenes are a group of phytoalexins that play an important role in grapevine (Vitis) basal immunity and can be induced by biotic and abiotic stresses. The levels of chlorophylls, the main pigments in plant cells, can also indicate the tolerance of plants to various stresses. Here, the response of different grapevine genotypes to UV-C radiation treatment was tested and the abundance of chlorophyll in the Hoe29 and Ke53 genotypes was observed to increase significantly within 6 h of UV-C treatment. Conversely, chlorophyll levels decreased markedly in the Augster Weiss and Müller-Thurgau genotypes. Furthermore, stilbene abundance increased substantially in the Hoe29, Ke53, Ke83 and Pinot Blanc genotypes, but increased only slightly in Augster Weiss and Müller-Thurgau. The expression of RESVERATROL SYNTHASE, which encodes a key enzyme in the stilbene synthesis pathway, increased in Hoe29, Ke53, Ke83 and Pinot Blanc following UV-C treatment, in a manner consistent with stilbene accumulation. In addition, we observed that reactive oxygen species (ROS) provide a key trigger in physiological responses and changes in secondary metabolite contents. In summary, the results from this study support a link between ROS, chlorophyll levels and genetic diversity for stilbene abundance in different grape genotypes, providing insights into mechanisms for plant physiological and biochemical responses and adaptations to stress.

芪类化合物（Stilbenes）是一类植物抗毒素（phytoalexins），在葡萄属（Vitis）植物的基础免疫中发挥重要作用，可由生物及非生物胁迫诱导产生。植物细胞中的主要色素——叶绿素（chlorophylls）的含量水平，同样可反映植物对各类胁迫的耐受性。本研究检测了不同葡萄基因型对UV-C辐射处理的响应：结果显示，Hoe29与Ke53基因型的叶绿素含量在UV-C处理后6小时内显著升高；与之相反，Augster Weiss与Müller-Thurgau基因型的叶绿素水平则显著下降。此外，Hoe29、Ke53、Ke83与Pinot Blanc基因型的芪类化合物含量大幅升高，而Augster Weiss和Müller-Thurgau基因型的芪类化合物含量仅小幅上升。编码芪类合成通路关键酶的白藜芦醇合酶（RESVERATROL SYNTHASE）基因的表达量，在UV-C处理后的Hoe29、Ke53、Ke83与Pinot Blanc中均出现上调，且该变化趋势与芪类化合物的积累一致。此外，本研究发现活性氧（ROS）是植物生理响应及次生代谢物含量变化的关键触发因子。综上，本研究结果证实了不同葡萄基因型中活性氧、叶绿素水平与芪类化合物含量的遗传多样性之间存在关联，为解析植物生理生化响应及逆境适应机制提供了新的见解。