Genome-wide maps of chromatin accessibility before and after NR2F2 knock down using ATAC-seq.

FOXA1 and GATA3 can remodel chromatin accessibility, we further explored what effect of NR2F2 would have on chromatin properties. ATAC-seq (Assay for Transposase Accessible Chromatin with high-throughput sequencing) is widely used to map chromatin accessibility genome-wide. Thus, We perform ATAC-seq without oestrogen stimulation before and after NR2F2 depletion.Covalent modifications are a main chromatin property.To test whether NR2F2 favoured histone modification deposition on chromatin, we profiled ChIP-Seq of H3K4me1, H3K4me3, and H3K27ac following NR2F2 depletion in oestrogen-starved MCF-7 cells to gain comprehensive histone medication landscape. Study of chromatin accessibility in WT and NR2F2 KO MCF-7 cells by deep sequencing using Illumina xten.

FOXA1与GATA3可重塑染色质开放状态，我们进一步探究了NR2F2对染色质特性的影响。ATAC-seq（Assay for Transposase Accessible Chromatin with high-throughput sequencing，转座酶可及性染色质高通量测序）被广泛用于全基因组范围的染色质开放状态图谱绘制。据此，我们在NR2F2敲除前后，于无雌激素刺激的条件下开展了ATAC-seq实验。共价修饰是染色质的核心特性之一。为验证NR2F2是否可促进组蛋白修饰在染色质上的沉积，我们对雌激素饥饿培养的MCF-7细胞在NR2F2敲除后，进行了H3K4me1、H3K4me3及H3K27ac的ChIP-seq测序分析，以获取全面的组蛋白修饰调控图谱。我们采用Illumina Xten平台开展深度测序，以此分析野生型（WT）与NR2F2基因敲除（KO）的MCF-7细胞的染色质开放状态。