WingsPElements_Rep2

These are the raw images associated with I. Dworkin and G. Gibson, “Epidermal growth factor receptor and transforming growth factor-beta signaling contributes to variation for wing shape in Drosophila melanogaster.,” Genetics, vol. 173, no. 3, pp. 1417–1431, Jul. 2006. doi:10.1534/genetics.105.053868 A subset of these were also used in doi:10.1534/genetics.110.125922 In brief 50 mutations (modified P-elements marked by a mini-white) were introduced into each of two wild type backgrounds (Samarkand and Oregon-R) via 10 generations of backcrossing. Flies were grown in a density, temperature and light controlled manner (see paper) allowed to eclose, sclerotize and then were stored in ethanol prior to dissection. Wings were dissected and then imaged. These are those images. The landmark data (for geometric morphometric analysis) can be found on DRYAD http://dx.doi.org/10.5061/dryad.8371 Please note: For simplicity I have uploaded all of the images on figshare into two seperate filesets (WingsPelements_Rep1 and WingsPelements_Rep2). You will want to have both of them! These are the raw images associated with I. Dworkin and G. Gibson, “Epidermal growth factor receptor and transforming growth factor-beta signaling contributes to variation for wing shape in Drosophila melanogaster.,” Genetics, vol. 173, no. 3, pp. 1417–1431, Jul. 2006. doi:10.1534/genetics.105.053868 A subset of these were also used in doi:10.1534/genetics.110.125922 In brief 50 mutations (modified P-elements marked by a mini-white) were introduced into each of two wild type backgrounds (Samarkand and Oregon-R) via 10 generations of backcrossing. Flies were grown in a density, temperature and light controlled manner (see paper) allowed to eclose, sclerotize and then were stored in ethanol prior to dissection. Wings were dissected and then imaged. These are those images. The landmark data (for geometric morphometric analysis) can be found on DRYAD http://dx.doi.org/10.5061/dryad.8371 Please note: For simplicity I have uploaded all of the images on figshare into two seperate filesets (WingsPelements_Rep1 and WingsPelements_Rep2). You will want to have both of them! Notes on naming conventions:GeneticBackground_BloomingtonStockNumber_genotype_sex_individual Where Genetic Background is either Ore (Oregon-R) or Sam (Samarkand). The two wild-type strains the mutations were introgressed into. BloomingtonStockNumber: Is the number for the strains from the Bloomington Stock center at the time of ordering. See the NamesOfMutants.csv for all the details about gene and allele info. genotype: whether the individuals emerged had the P-element insertion (p) meaning they are mutant, or they had white (w) eyes (without the insertion) and were thus wild type for the gene in question. sex: male or female individual. The individual fly. So<br>Sam_02513_w_M_00010.jpg would be from the cross with the insertion 02513 (allele of argos) in the Samarkand background, but without the mutant allele of argos (as they are "w" for genotype) and male. As the replicates were done seperately, they were accidently not coded with rep1 and rep2 in the image names, so I kept them seperate. However, they do represent distinict individuals grown seperately. Notes on all images: I did not notice until the discs with images were copied that one of the two undergrads who digitally captured wings, would only take a single image, when two complete wings were in the image. Therefore , for some lines the numbers appear small. What I manually had to do was go through each folder and look at the thumbnails to see which images I needed to make duplicates of prior to making TPS files for morphometrics. Depending on your goals a similar approach may be necessary. Links http://dx.doi.org/10.1534/genetics.105.053868

本数据集关联的原始图像来自I. Dworkin与G. Gibson发表于《Genetics》2006年7月第173卷第3期第1417-1431页的论文《表皮生长因子受体与转化生长因子-β信号通路参与黑腹果蝇（Drosophila melanogaster）翅膀形状的变异调控》，其DOI为10.1534/genetics.105.053868。该数据集的部分图像也曾用于DOI为10.1534/genetics.110.125922的研究。

简言之，研究人员通过10代回交（backcrossing），将50株以mini-white标记的修饰P因子（P-element）突变体分别导入两个野生型遗传背景（萨马尔坎德Samarkand与俄勒冈-R Oregon-R）。果蝇在严格控制密度、温度与光照的条件下培养（详细方法参见原论文），待其羽化（eclose）、表皮硬化（sclerotize）后，于解剖前以乙醇保存样本。随后研究人员解剖果蝇翅膀并进行成像，本数据集即包含这些原始图像。用于几何形态计量学分析（geometric morphometric analysis）的地标数据可在DRYAD数据库获取，链接为http://dx.doi.org/10.5061/dryad.8371。

为简化获取流程，本数据集已在figshare平台上传为两个独立文件集（WingsPelements_Rep1与WingsPelements_Rep2），请同时下载这两个文件集。

### 图像命名规则
图像命名遵循以下格式：GeneticBackground_BloomingtonStockNumber_genotype_sex_individual。其中：
1. Genetic Background：取值为Ore（代表俄勒冈-R Oregon-R野生型品系）或Sam（代表萨马尔坎德Samarkand野生型品系），即导入突变体的两个亲本野生型品系；
2. BloomingtonStockNumber：订购时布卢明顿果蝇保藏中心（Bloomington Stock Center）分配的品系编号；
3. 详细的基因与等位基因信息请参阅NamesOfMutants.csv文件；
4. genotype：表示个体是否携带P因子插入突变，取值为p（突变型，携带插入片段）或w（野生型，复眼为白色，未携带插入片段，对应目标基因的野生型）；
5. sex：个体性别，取值为male（雄性）或female（雌性）；
6. 最后一段为果蝇个体编号。

例如，Sam_02513_w_M_00010.jpg对应的样本为：在萨马尔坎德遗传背景下导入编号02513的突变体（argos基因的等位基因），但未携带argos的突变等位基因（genotype为w，即野生型），且为雄性个体。由于两次实验重复独立开展，但图像命名未标注rep1与rep2，因此将数据拆分为两个独立文件集以区分重复样本，二者对应独立培养的不同个体。

### 图像补充说明
在复制图像存储磁盘时，研究人员才发现其中一名负责数字化拍摄翅膀的本科生，仅为每张包含完整双翅的图像拍摄了单张照片，因此部分品系的图像数量偏少。为制作用于形态计量分析的TPS文件（TPS file），研究人员手动遍历每个文件夹，通过缩略图确认需要复制的图像。若您有类似研究需求，也可采用相同的处理方式。

原论文链接：http://dx.doi.org/10.1534/genetics.105.053868