The role of the microbiome in the gastrointestinal colonization with carbapenemase-producing Klebsiella pneumoniae

In this study we examine a murine model of gastrointestinal colonization with OXA-162-carbapenemase and CTX-M-15 extended-spectrum beta-lactamase producing Klebsiella pneumoniae following the administration of ampicillin, ceftazidime and ciprofloxacin. We investigated the effects of various antibiotic treatments on the level of gastrointestinal colonization with the carbapenemase-producing Klebsiella, on the amount of detectable resistance genes (CTX-M-15 and OXA-162), the horizontal gene transfer, and on the composition dysbiosis and diversity of the microbiome by analysing the feaces

本研究针对经氨苄西林（ampicillin）、头孢他啶（ceftazidime）与环丙沙星（ciprofloxacin）给药后，产OXA-162碳青霉烯酶（OXA-162-carbapenemase）及CTX-M-15超广谱β-内酰胺酶（CTX-M-15 extended-spectrum beta-lactamase）的肺炎克雷伯菌（Klebsiella pneumoniae）胃肠道定植的小鼠模型展开考察。本研究通过粪便样本分析，探究了多种抗生素治疗方案对该产碳青霉烯酶克雷伯菌胃肠道定植水平、可检测耐药基因（CTX-M-15与OXA-162）含量、水平基因转移（horizontal gene transfer）水平，以及微生物组（microbiome）的组成失调与多样性的影响。