A study of stem cell marker lineage marker gene expression and clonal expansion in TGFβR2iSMC-Apoe mice Chen et al. Related to Fig 4

Characterization of TGFβR2iSMC-Apoe-/- mice after 1 month of high cholesterol high fat by histology, imaging mass cytometry (IMC), histoCAT, CyTOF, and immunocytochemistry. Aortic smooth muscle cells from TGFβR2iSMC-Apoe-/- express stem cell markers (CD105, CD73, CD90, Sca-1, CD44), mesenchymal cell markers (Osteopontin, Aggrecan, Adiponectin). To show clonal origin of smooth muscle cell-derived mesenchymal cells in TGFβR2iSMC-Apoe mice-/-, we replaced mTmG reporter strain TGFβR2iSMC-Apoe mice generating Myh11CreERT2;Tgfbr2f/f;Apoe-/-;Confettif/f and Myh11CreERT2;Apoe-/-:Confettif/f (control) lines.

本研究通过组织学、成像质谱流式细胞术（imaging mass cytometry, IMC）、histoCAT、飞行时间质谱流式细胞术（CyTOF）与免疫细胞化学技术，对高胆固醇高脂肪饮食饲喂1个月后的TGFβR2iSMC-Apoe^-/-小鼠开展表征分析。该小鼠的主动脉平滑肌细胞可表达干细胞标志物（CD105、CD73、CD90、Sca-1、CD44）与间充质细胞标志物（骨桥蛋白、聚集蛋白聚糖、脂联素）。为阐明TGFβR2iSMC-Apoe^-/-小鼠中平滑肌细胞来源间充质细胞的克隆起源，我们对该品系小鼠进行遗传改造，替换其mTmG报告基因位点，成功构建了Myh11CreERT2;Tgfbr2^f/f;Apoe^-/-;Confettif/f与Myh11CreERT2;Apoe^-/-:Confettif/f（对照组）两种实验品系。