RNASeq of Drosophila intestinal progenitor cells upon Notch knockdown or microbiome alterations. RNASeq of Drosophila intestinal progenitor cells upon Notch knockdown or microbiome alterations

We sequenced the transcriptome of the proliferative progenitor cells of the intestine to determine how the microbiome was affecting growth in wild-type intestines and those with Notch-deficient tumors Overall design: A total of 6 conditions and 17 samples were analyzed. For each condition, we used 3 biological replicates, except for the Gfesgts where an n=2 was used due to the loss of one sample upon RNA extraction.

本研究对肠道增殖祖细胞的转录组进行测序，旨在探究微生物组对野生型肠道及携带Notch缺陷型肿瘤的肠道生长的影响。实验设计概述：本研究共分析6种实验条件下的17份样本。除Gfesgts组外，其余各组均设置3次生物学重复；Gfesgts组因RNA提取过程中丢失1份样本，故仅设置2次生物学重复（n=2）。