Additional file 1: of Levels of activated platelet-derived microvesicles in patients with soft tissue sarcoma correlate with an increased risk of venous thromboembolism

Representative fluorescence-activated cell sorting (FACS) dot plots. A. Forward and side scatter of isolated microvesicles stained with Fluorescein (FITC) Annexin V and Phycoerythrin (PE) anti-CD61 as well as TruCOUNT calibrating beads; MV = microvesicle gate. B. Events within MV-gate. Q1 = buffer / background. Q2 = Annexin V-positive and CD61-positive microvesicles. Q3 = Annexin V-positive microvesicles. (ZIP 121 kb)

代表性荧光激活细胞分选（Fluorescence-activated cell sorting, FACS）散点图。A. 针对经荧光素（Fluorescein, FITC）标记的膜联蛋白V（Annexin V）与藻红蛋白（Phycoerythrin, PE）标记的抗CD61抗体染色，且添加了TruCOUNT校准微球的分离微囊泡，绘制的前向散射光与侧向散射光散点图；MV = 微囊泡设门区域。B. 微囊泡设门区域内的检测事件。Q1 = 缓冲液本底信号。Q2 = 膜联蛋白V阳性且CD61阳性的微囊泡。Q3 = 膜联蛋白V阳性的微囊泡。（ZIP 压缩包，121 kb）