RNA-seq analysis of Drosophila melanogaster midgut during Pseudomonas entomophila infection

This study investigates transcriptional changes in the adult Drosophila melanogaster midgut upon oral infection with the entomopathogen Pseudomonas entomophila for 20 hours. Midgut samples were collected from 5–7 day old flies under two conditions: uninfected control and P. entomophila-infected. genotype – NP1Gal4>UAS-Luciferase-RNAi本研究探究了成年黑腹果蝇（Drosophila melanogaster）中肠经口服昆虫病原细菌嗜虫假单胞菌（Pseudomonas entomophila）感染20小时后的转录水平变化。实验从5至7日龄的果蝇中采集中肠样本，设置两种实验条件：未感染对照组与嗜虫假单胞菌感染组。实验所用果蝇的基因型为NP1Gal4>UAS-荧光素酶RNA干扰（Luciferase-RNAi）。