Mechanically stimulated fibroblast from different fetal mouse tissues using Affy MOE430 chip set

In order to test the hypothesis that fibroblasts from different tissues are phenotypically distinct from one another, we have subjected tendon, skin and corneal fibroblasts of fetal mouse to mechanical stimulation by fluid flow and analyzed the transcriptional responses of the cells using Affymetrix MOE430 chip set containing two arrays MOE430A and MOE430B. Keywords: cell type comparison, stress response The experiment is done using Affymetrix MOE430 chip set containing two arrays MOE430A - platform GPL339 and MOE430B - platform MOE340. Three biological replicates for tendon, skin and corneal fibroblasts subjected to mechanical stimulation as well as not stimulated (control) were analyzed yielding 18 samples per chip (36 in total).

为验证"不同组织来源的成纤维细胞表型存在差异"这一假说，我们对胎鼠的肌腱、皮肤及角膜成纤维细胞施加流体流动介导的机械刺激，并采用搭载MOE430A与MOE430B两张阵列的Affymetrix MOE430芯片组分析细胞的转录响应。
关键词：细胞类型比较、应激响应
本实验所用的Affymetrix MOE430芯片组包含两张阵列，其中MOE430A对应平台GPL339，MOE430B对应平台MOE340。针对接受机械刺激与未接受刺激（对照组）的肌腱、皮肤及角膜成纤维细胞，我们各设置3次生物学重复，最终每张芯片对应18个样本，总计36个样本。