Table_1_Changes in the Salivary Proteome Associated With Canine Pyometra.DOC

The present study evaluated for the first time changes in the saliva proteome in bitches with pyometra through a high-throughput quantitative proteomic analysis. The aims were to explore whether saliva composition could reflect the physiopathological changes occurring in canine pyometra and to identify potential biomarkers of the disease. Saliva samples from six healthy (H) and six bitches with pyometra (P) were analyzed using tandem mass tags–based approach. Additionally, 15 samples were used for the validation of changes in haptoglobin (Hp) concentration in saliva of dogs with pyometra. Proteomic analysis quantified 707 proteins in saliva. Comparison of the two groups revealed 16 unique proteins significantly modulated in saliva, with S100A calcium-binding protein 12 (S100A12), vimentin, and Hp the most up-regulated in canine pyometra. According to PANTHER (Protein Analysis Through Evolutionary Relationships) classification tool, these proteins are mainly related to proinflammatory mediators, acute-phase proteins, and sepsis. In conclusion, it can be stated that there are changes in various proteins in saliva in canine pyometra reflecting different physiopathological changes occurring in this disease. These proteins could be a source of potential non-invasive biomarkers for this disease that should be confirmed in future studies.

本研究首次通过高通量定量蛋白质组学分析，评估了患有子宫蓄脓的母犬唾液蛋白质组的变化。研究旨在探讨唾液成分是否能够反映犬类子宫蓄脓发生的生理病理变化，并识别疾病的潜在生物标志物。采用串联质谱标签技术对六只健康犬（H）和六只患有子宫蓄脓的犬（P）的唾液样本进行分析。此外，还使用了15个样本来验证患有子宫蓄脓的犬唾液中触珠蛋白（Hp）浓度的变化。蛋白质组学分析在唾液中量化了707种蛋白质。两组的比较揭示了16种在唾液中显著调节的独特蛋白质，其中S100A钙结合蛋白12（S100A12）、波形蛋白和Hp在犬类子宫蓄脓中表达上调最为显著。根据PANTHER（通过进化关系分析蛋白质）分类工具，这些蛋白质主要与促炎介质、急性期蛋白和败血症相关。综上所述，可以断言，在犬类子宫蓄脓中，唾液中的多种蛋白质发生了变化，这些变化反映了该疾病发生的不同生理病理变化。这些蛋白质可能是该疾病潜在的无创生物标志物的来源，应在未来的研究中得到证实。