Human tonsil with 17 channels

This is an image of a human tonsil collected on the Rarecyte Orion. This is a widefield spectral-unmixing microscope with a 20x (0.75) dry objective. The slide was stained with 15 antibodies, Hoechst, and autofluorescence was calculated (AF1) . The channels are: 01 Hoechst 02 AF1 03 CD31_Argo515 04 CD11c_Argo555L 05 CD68_Argo535 06 CD163_Argo580L 07 CD20_Argo660L 08 CD4_Argo572 09 CD8a_Argo602 10 CD45RO_Argo624 11 PD-L1_Argo662 12 FOXP3_Argo686 13 LAG3_Argo730 14 PD-1_Argo760 15 Ki-67_Argo784 16 CD45_Argo810 17 S100_Argo845本数据集为采集于Rarecyte Orion设备的人类扁桃体组织成像图。该设备为搭载20×（0.75）干式物镜的宽场光谱拆分显微镜。本次实验所用玻片经15种抗体及赫斯特（Hoechst）染色，并计算了自发荧光（AF1）信号。各成像通道信息如下： 01 赫斯特（Hoechst） 02 自发荧光（AF1） 03 CD31_Argo515 04 CD11c_Argo555L 05 CD68_Argo535 06 CD163_Argo580L 07 CD20_Argo660L 08 CD4_Argo572 09 CD8a_Argo602 10 CD45RO_Argo624 11 PD-L1_Argo662 12 FOXP3_Argo686 13 LAG3_Argo730 14 PD-1_Argo760 15 Ki-67_Argo784 16 CD45_Argo810 17 S100_Argo845