Gene expression analysis of hyper active mutant SPORTS rat [muscle]

To get insight into the genetic characteristics of hyper active mutant line of rat, SPORTS, and the effect of exercise on gene expression, we compared gene expression profiles of exercised SPORTS rat, sedentary SPORTS rat, and sedentary wild type rat. Using RNA extracted from the muscle of these rats, we performed microarray analysis. Subsequent GO analyses revealed that genes belonging to muscle development and glycolysis were upregulated in exercised SPORTS rat compared to sedentary SPORTS rat, and genes related to coagulation were upreguated in sedentary SPORTS rat compared to wild type rat. These results were consistent with phenotypes, such as hyper activity and thrombotic tendency, which were reported for SPORTS rat. Gene expression was measured using RNA prepared from the skeletal muscle of exercised SPORTS rat, sedentary SPORTS rat, and sedentary wild type rat. The background strain of SPORTS rat is Wistar, and the wild type rat, we used in this experimet, is also Wistar strain.

为深入解析SPORTS多动突变大鼠品系的遗传特征，以及运动对基因表达的调控效应，我们对比了运动组SPORTS大鼠、安静组SPORTS大鼠与安静组野生型大鼠的基因表达谱。我们从上述大鼠的肌肉组织中提取RNA，开展了基因芯片（microarray）分析。后续的基因本体（Gene Ontology, GO）分析显示：相较于安静组SPORTS大鼠，运动组SPORTS大鼠中参与肌肉发育与糖酵解过程的基因呈上调表达；相较于野生型大鼠，安静组SPORTS大鼠中与凝血相关的基因呈上调表达。上述结果与此前报道的SPORTS大鼠所具有的多动表型及血栓倾向特征相一致。本研究通过提取运动组SPORTS大鼠、安静组SPORTS大鼠与安静组野生型大鼠的骨骼肌RNA，完成了基因表达量的检测。SPORTS大鼠的背景品系为Wistar，本研究中使用的野生型大鼠同样为Wistar品系。