Cre-mediated site-specific translocation between nonhomologous mouse chromosomes.
收藏PubMed Central1995-08-01 更新2026-05-02 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC41342/
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资源简介：
Chromosome rearrangements, such as large deletions, inversions, or translocations, mediate migration of large DNA segments within or between chromosomes, which can have major effects on cellular genetic control. A method for chromosome manipulation would be very useful for studying the consequences of large-scale DNA rearrangements in mammalian cells or animals. With the use of the Cre-loxP recombination system of bacteriophage P1, we induced a site-specific translocation between the Dek gene on chromosome 13 and the Can gene on chromosome 2 in mouse embryonic stem cells. The estimated frequency of Cre-mediated translocation between the nonhomologous mouse chromosomes is approximately 1 in 1200-2400 embryonic stem cells expressing Cre recombinase. These results demonstrate the feasibility of site-specific recombination systems for chromosome manipulation in mammalian cells in vivo, breaking ground for chromosome engineering. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1995-08-01
