Sex-Biased Gene Expression and Sequence Conservation in Pacific and Atlantic Salmon Lice (Lepeophtheirus salmonis)

This study investigates sex-biased gene expression between populations of Atlantic and Pacific salmon lice, Lepeophtheirus salmonis. Two Atlantic L. salmonis populations were previously used for an array study (GSE56024) while a third dataset using Pacific L. salmonis was novel. Using all three populations, a consensus-based, meta-analysis approach was used to identify sex-biased and sex-specific genes. Two separate experiments were conducted, one for Atlantic and one for Pacific salmon lice. As the Atlantic data has been previously published for other comparisons (GSE56024), only the Pacific data is uploaded here. Lice from three populations (2 in the Atlantic and 1 in the Pacific) were collected for in vitro bioassay analysis using emamectin benzoate. After 24hrs, lice were collected as per treatment protocol below. Males and females from all populations were compared separately before forming a consensus probe list of sex-biased genes concordantly expressed across all three populations. Please note that each raw data file contains three or four 'block' data and each block data correspond to individual sample raw data. Therefore, each raw data file contains raw data for 3-4 samples (as indicated in the description field).

本研究旨在探究大西洋鲑虱与太平洋鲑虱（*Lepeophtheirus salmonis*）不同种群间的性别偏向性基因表达模式。此前已有两项大西洋鲑虱种群的转录组数据被应用于芯片阵列研究（GSE56024），而基于太平洋鲑虱的第三项数据集为全新生成的数据。本研究整合全部三类种群的相关数据，采用基于共识的元分析方法，以鉴定具有性别偏向性及性别特异性的基因。本研究开展了两组独立实验，分别针对大西洋鲑虱与太平洋鲑虱；由于大西洋鲑虱的相关数据此前已用于其他比对分析（GSE56024），因此本次仅上传太平洋鲑虱的数据集。研究人员从三个种群（其中大西洋种群2个、太平洋种群1个）中采集鲑虱样本，使用甲氨基阿维菌素苯甲酸盐（emamectin benzoate）开展体外生物测定分析，样本培养24小时后按照下述处理流程采集鲑虱。在构建覆盖全部三个种群的性别偏向性基因共识探针列表之前，研究人员先对所有种群的雄性与雌性个体分别进行单独比对分析。请注意，每个原始数据文件包含3至4组“区块”数据，每组区块数据对应单一样本的原始数据，因此每个原始数据文件包含3-4个样本的原始数据（具体信息可参见描述字段）。