Fungal Diversity Drives Non-linear Trajectories of Soil Multi-functionality during Alpine Grassland Restoration

We collected soil samples from alpine grasslands on the eastern Qinghai-Tibet Plateau across a restoration chronosequence of 1, 5, 7, 13, and 20 years, with a total of 30 samples. Soil microbial diversity was characterized using high-throughput amplicon sequencing targeting the bacterial 16S V4 region and the fungal ITS region, allowing the assessment of bacterial and fungal community composition. Microbial alpha diversity was quantified using richness metrics, which were used to describe variations in bacterial and fungal diversity across different restoration stages and their relationships with soil multifunctionality.

本研究于青藏高原东部高寒草地采集土壤样本，覆盖1、5、7、13及20年的恢复演替序列（restoration chronosequence），共获取30份样品。本研究采用靶向细菌16S V4区与真菌ITS区的高通量扩增子测序（high-throughput amplicon sequencing）表征土壤微生物多样性，以此解析细菌与真菌的群落组成。研究通过丰富度指数（richness metrics）定量微生物α多样性（alpha diversity），用于阐释不同恢复阶段下细菌与真菌多样性的变化特征，及其与土壤多功能性（soil multifunctionality）之间的关联。