Whole genome bisulfite sequencing of ros1-1 mutant and C24 wild-type (WT)

Using whole genome bisulfite sequencing to provide single-base resolution of DNA methylation status in ros1-1 mutant and C24 WT Overall design: 2 samples examined: C24 wild type with RD29A-LUC transgene, ros1-1 mutant (in C24 background with RD29A-LUC transgene)

本研究采用全基因组亚硫酸氢盐测序（whole genome bisulfite sequencing）技术，获取ros1-1突变体（ros1-1 mutant）与C24野生型（C24 WT）的DNA甲基化状态单碱基分辨率信息。实验整体设计：共检测2个样本：携带RD29A-LUC转基因（RD29A-LUC transgene）的C24野生型，以及处于携带RD29A-LUC转基因的C24遗传背景下的ros1-1突变体。