CIL:18441, damselfly, flight muscle cell. In Cell Image Library

Most images show fractures through the outer membrane of mitochondria and SR/T tubules of dyads nestled in long grooves of the mitochondrial surface. All leaflets of the membranes appear in the images. This image is part of the group image CFA00512. TEM magnifications is not available for this image. The negative film was scanned at LBL then reduced in size for upload. Scan step size is 6.3 micron, then reduced in size 4X for upload, for an effective pixel size of 25 micron. Technical details: Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer. pH 7.2. stored at 4C for several days. Muscle dissected out. Infiltrated in 30% glycerol in water. Frozen in liquid nitrogen cooled Freon, fractured in Balzers 400 Freeze-fracture, shadowed with platinum at 45o angle. Replicas were cleaned with Clorox (to remove tissue and with either sulfuric acid or NaOH to try removing the tracheolae. Replicas examined in a Philips 410 Microscope. Reference for these images:
Takekura, H. and Franzini-Armstrong C. The structure of Ca2+ release units in arthropod body muscle indicates an indirect mechanism for excitation-contraction coupling. Biophys. J. 83, 83, 2742-2753, 2002.

多数图像呈现了线粒体外膜的断裂面，以及嵌于线粒体表面长凹槽内的二联体（dyads）的肌质网（Sarcoplasmic Reticulum, SR）/横小管（Transverse Tubule, T tubules）结构。图像中可清晰观察到所有膜小叶层。
本图像隶属于图像组CFA00512。本图像未提供透射电子显微镜（Transmission Electron Microscope, TEM）的放大倍数信息。
原始负片于劳伦斯伯克利国家实验室（Lawrence Berkeley National Laboratory, LBL）完成扫描，上传前进行了尺寸压缩。扫描步长为6.3微米，上传前将图像尺寸缩小为原尺寸的1/4，最终有效像素尺寸为25微米。
技术细节：去除头尾部，将胸部对半切开后置于0.1M二甲胂酸盐（cacodylate）缓冲液配制的3%戊二醛（glutaraldehyde）溶液中固定，pH值为7.2，于4℃保存数日。分离肌肉组织后，将其浸润于30%甘油水溶液中，采用液氮冷却的氟利昂（Freon）进行冷冻，随后在Balzers 400型冷冻断裂装置中完成冷冻断裂，以45°角喷镀铂进行重金属阴影复型。使用高乐氏漂白剂（Clorox）清洗复型膜以去除组织残留，并使用硫酸（sulfuric acid）或氢氧化钠（Sodium Hydroxide, NaOH）尝试去除气管结构。复型膜在飞利浦（Philips）410型显微镜下进行观测。
本图像相关参考文献：Takekura H、Franzini-Armstrong C. 节肢动物体肌内钙释放单元的结构表明兴奋-收缩偶联存在间接机制. 《生物物理杂志》（Biophysical Journal, Biophys. J.），2002年，第83卷，第2742-2753页。