Single-cell RNA-Seq analysis reprogramming of T to ITNK

To further decipher the cell fate continuum during reprogramming of T cells to ITNKs, we investigated the gene expression dynamics of T and ITNK cells from a CB sample at single-cell level using micro-well based scRNA-seq technology at different time points post BCL11B ablation T cells that were electroplated with PX458-BCL11B at day 0 (2263 cells), day 5 (1565 cells), day 10 (498 cells), day 15 (204 cells), and day 20 (418 cells), based on sc-RNA seq analysis.

为进一步解析T细胞重编程为固有样自然杀伤T细胞（ITNKs）过程中的细胞命运连续体，我们采用基于微孔的单细胞RNA测序（scRNA-seq）技术，对取自一份脐带血（CB）样本、经PX458-BCL11B电转染以敲除BCL11B的T细胞，在电转染后第0天（2263个细胞）、第5天（1565个细胞）、第10天（498个细胞）、第15天（204个细胞）及第20天（418个细胞）的单细胞基因表达动态开展分析，所有分析均基于单细胞RNA测序结果。