Prostate H3K4me3 ChIP-seq

Exposure to pesticide chlordecone is associated with high risk of prostate cancer.We hypothesised that changes in histone H3K4me3 could be involved in pathological processes in prostate induced by chlordecone. We exposed mice to chlordecone during embryonic development (E6.5-E15.5) and we analysed F1 progeny male epigenetic H3K4me3 marks in prostate tissue, as well as H3K4me3 in the prostate of F3 progeny to reveal the transgenerational effects resulting from the ancestral exposure to CD. We performed ChIP using H3K4me3 commercial antibody (Merck Millipore, 07-473) and pooled prostate tissues from 4 mice. DNA-protein complex was immunoprecipitated, eluted and DNA purified. The DNA from ChIP, samples of F1 animals: 1. PS1 control 2. PS2 control 3. PS3 chlordecone 4. PS4 chlordecone 5. PS5 input Four libraries (H3K4me3 ChIP) were prepared from prostate of F3 mice. Two replicates from control-derived mice (C5, C7) and two replicates from CD-derived mice (K4, K11).

农药十氯酮（chlordecone）暴露与前列腺癌的高发风险存在显著关联。本研究假设组蛋白H3K4me3的修饰变化可能参与十氯酮诱导的前列腺病理进程。本研究在小鼠胚胎发育阶段（E6.5-E15.5）对其进行十氯酮暴露处理，随后分别分析F1代雄性子代前列腺组织中的表观遗传标记H3K4me3，以及F3代小鼠前列腺组织的H3K4me3水平，以揭示亲代十氯酮暴露所产生的跨代遗传效应。本研究使用商品化H3K4me3抗体（Merck Millipore, 07-473），并将4只小鼠的前列腺组织混合后开展染色质免疫沉淀（ChIP）实验：对DNA-蛋白质复合物进行免疫沉淀、洗脱及DNA纯化操作。本次ChIP所得的DNA样本对应F1代小鼠分组如下：1. PS1对照组 2. PS2对照组 3. PS3十氯酮暴露组 4. PS4十氯酮暴露组 5. PS5 输入对照（Input）。本研究从F3代小鼠的前列腺组织中制备了4个H3K4me3 ChIP文库，其中2个生物学重复来自对照组小鼠（C5、C7），另外2个生物学重复来自十氯酮暴露亲代的子代小鼠（K4、K11）。