Expression data from Rattus norvegicus cornea. Rattus norvegicus

Inflammation is a key component of pathological angiogenesis. Here we induce cornea neovascularisation using sutures placed into the cornea, and sutures are removed to induce a regression phase. We used whole transcriptome microarray to monitor gene expression profies of several genes Overall design: This was a time course study conprising two arms. In the suture IN arm, sutures were maintained in the cornea after the 0h time point, while suture OUT arm is where both sutures were removed from the cornea at the 0h timpoint. 0h timepoint is when sprouts were 3/4 the distance from the pre-existing limbal vessels towards the sutures. Microarrays were performed at 0h, 24h suture IN and 24h suture OUT. Non sutured eyes from separate animals were used as controls. At each time point except for 0h=3 rats, the rest of the timepoints had four rats each.

炎症是病理性血管生成（pathological angiogenesis）的关键组成部分。本研究通过将缝线植入角膜以诱导角膜新生血管化（corneal neovascularisation），并通过移除缝线启动消退阶段。我们采用全转录组微阵列（whole transcriptome microarray）技术监测多个基因的表达谱。实验整体设计：本研究为时间进程实验，包含两个处理组别。缝线保留组（suture IN arm）：在0小时时间点后，缝线仍留存于角膜内；缝线移除组（suture OUT arm）：于0小时时间点将角膜内的两根缝线全部移除。0小时时间点的判定标准为：新生血管芽从已存在的角膜缘血管向缝线延伸的距离达到总距离的3/4。实验分别在0小时、缝线保留组24小时以及缝线移除组24小时三个时间点开展微阵列检测。以独立于实验组的未植入缝线的大鼠眼部组织作为对照样本。各时间点的样本量如下：0小时时间点使用3只大鼠，其余时间点均各使用4只大鼠。