Identification of genome wide Hoxa9 binding sites in primary murine and human AML cells. Identification of genome wide Hoxa9 binding sites in primary murine and human AML cells

Relative overexpression of HOXA9 is a key feature of aggressive AML (acute myeloid leukemia). Here we determined genome wide binding sites of Hoxa9 in primary murine cells transformed by Hoxa9 and in a human AML cell line. In addition global H3K4 monomethylation and H3K27acetylation levels were determined in cells transformed by an inducible Hoxa9-ER construct in Hoxa9-active conditions and 72h after Hoxa9 was inactivated.

同源盒A9（HOXA9）的相对过表达是侵袭性急性髓系白血病（acute myeloid leukemia, AML）的关键特征。本研究对经HOXA9转化的原代小鼠细胞以及人急性髓系白血病细胞系中的HOXA9全基因组结合位点进行了鉴定。此外，本研究还检测了经诱导型HOXA9-ER融合基因构建体转化的细胞，在HOXA9激活状态以及HOXA9失活72小时后的全基因组H3K4单甲基化（H3K4 monomethylation）与H3K27乙酰化（H3K27acetylation）水平。