CIL:909

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 68,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. Stereo pair with CIL:915.本数据集的样本为兔腰大肌骨骼肌纤维，将其维持于恒长状态，并通过浸入甘油诱导肌纤维僵直。随后将纤维置于Heuser型冷冻压榨仪中，通过与液氦冷却的铜块接触实现快速冷冻；接着在Balzers 400型冷冻断裂仪内完成冷冻蚀刻操作。之后以24°角采用铂碳（Pt-C）对样本进行旋转复型，再置于加速电压为100 keV的JEOL 100CX2型透射电子显微镜下成像，图像以68000倍的放大倍率记录于柯达4489胶片上。实验拍摄了倾角为±5°的立体图像对，使用尼康Coolscan 9000ED扫描仪以20微米的像素间距对胶片进行扫描，该立体图像对的编号为CIL:915。