File S1 - Microbial Dysbiosis in Colorectal Cancer (CRC) Patients

This file contains description of the whole cohort (Table S1) from which subgroup of patients for microbiota analysis has been selected. The subgroup's characteristics are similar to those of the whole cohort. Additional information on pyrosequencing analyses (Tables S2 and Table S3), primers selected for qPCR of dominant and sub dominant bacteria families (Table S4) as well as correlation with patients' characteristics such as BMI, diet regimen (Figure S1) are given. Additional illustration of bacterial species abundance belonging to the phylogenetic core differentiates cancer patients and healthy individuals (Figure S2) and rarefaction analysis of the pyrosequencing reveals validity of the results (Figure S3). For mucosa-adherent bacteria analysis, characterization of probes for targeting the Bacteroides genus in mucosa samples are indicated and sequences of amplification products from mucosa are indicated (Figure S4); Bacteroides 16S rRNA and human Albumin genes assessed by Gel electrophoresis are shown on normal and tumoral mucosa (Figure 5). (DOC)本文件包含全部队列（附表S1）的相关描述，本研究用于微生物组分析的患者亚组即从该队列中筛选得到，且该亚组的临床特征与全部队列基本一致。本文件还提供了焦磷酸测序分析的补充信息（附表S2、S3）、用于优势及次优势细菌家族定量聚合酶链反应（qPCR）的引物序列信息（附表S4），以及患者特征（如体重指数BMI、饮食方案）与上述指标的相关性分析结果（附图S1）。另有附图展示了系统发育核心菌群的物种丰度情况，以区分癌症患者与健康个体（附图S2）；同时通过焦磷酸测序的稀疏性分析验证了实验结果的可靠性（附图S3）。针对黏膜黏附细菌的分析，本文件说明了用于靶向黏膜样本中拟杆菌属（Bacteroides）的探针设计信息，并提供了黏膜样本扩增产物的序列详情（附图S4）；此外还展示了通过凝胶电泳检测得到的正常与肿瘤黏膜组织中拟杆菌16S rRNA及人白蛋白基因的电泳结果（图5）。(DOC)