Characterization of the crosstalk between Notch signaling and hypoxia

The Notch signaling pathway regulates several differentiation and developmental processes. Reduction of oxygen availability, a condition referred to as hypoxia, impacts on the Notch response. Here we made use of RNA-Seq and ChIP-Seq analysis to investigate the Notch/hypoxia crosstalk at genomic level. Mouse pre-T cells (Beko) were treated with the DMOG or kept under low oxygen concentration to induce hypoxia. Effects on gene expression were investigated by RNA-Seq and RBPJ occupancy was analyzed by ChIP-Seq.

Notch信号通路（Notch signaling pathway）可调控多种分化与发育进程。氧可利用度降低，即缺氧（hypoxia）状态，可对Notch信号应答产生影响。本研究借助RNA测序（RNA-Seq）与染色质免疫沉淀测序（ChIP-Seq）技术，在基因组层面探究Notch信号与缺氧的信号串扰。实验中将小鼠前T细胞（Beko）或经DMOG处理，或置于低氧浓度环境以诱导缺氧状态。随后通过RNA-Seq分析其对基因表达的影响，并通过ChIP-Seq检测RBPJ的染色质结合占有率。