RNA-seq of GFP+ (Myod-lineage) progenitor cells versus Tomato+ progenitor cells from inguinal WAT of Myod-driven mTmG reporter mice. RNA-seq of GFP+ (Myod-lineage) progenitor cells versus Tomato+ progenitor cells from inguinal WAT of Myod-driven mTmG reporter mice

In our study, for identify the Myod-lineage precursor cells from inguinal WAT, the Myod-Cre; mTmG reporter mice were used. GFP+ (Myod-lineage) SVF progenitor cells from inguinal WAT (pooled from 3 animals) were sorted by FACS sorting, and plate in cell culture for total RNA isolation; as comparison, the Tomato+ SVF progenitor cells from the same tissue (pooled from 3 animals) were sorted by FACS sorting for total RNA isolation.

本研究中，为了从腹股沟白色脂肪组织（inguinal white adipose tissue，WAT）中鉴定并分离肌分化因子（Myod）谱系前体细胞，我们使用了Myod-Cre;mTmG报告基因小鼠。将合并自3只小鼠的该脂肪组织来源的GFP阳性（Myod谱系）基质血管组分（stromal vascular fraction，SVF）前体细胞，通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS）进行分选后接种于细胞培养体系，以提取总RNA；作为对照，我们同样分选了合并自3只小鼠的同一脂肪组织中的Tomato阳性基质血管组分前体细胞，经FACS分选后用于总RNA提取。