Brain RNA-seq: Esomeprazole/stress interactions.

Purpose: The goals of this study were to evaluate the effects of acid suppression on the brain transcriptomer in a murine model of stress. In addition, the effects of the proton pump inhibitor (PPI), esomeprazole, on the brain transcriptome were evaluted in the presence or absence of stress. Methods: Male C57BL/6 mice were subjected to three days of stress by hypothermic immobilization or control environment for three hours daily and either esomeprazole 2 mg/kg or saline by intraperitoneal injection daily. Standard RNA-seq methods were employed to assess mRNA expression in the hippocampus and frontal cortex. Results: There was no effect of stress or esomeprazole on the frontal cortex transcriptome. Stress had no impact on the hippocampus transcriptome, but the addition of esomeprazole induced differential expression of 124 genes, many of which are involved in cognitive and behavior pathways. Conclusions: Further studies are needed to evaluate the associations between microbiota, host gene expression, the abundance of CNS neurocognitive modulators, and their impact on cognition and behavior. mRNA from hippocampus and frontal cortex was profiled using Illumina HiSeq 2000 (2x151). Tissue was generated from 4 groups of 6 male C57BL/6 mice: 1) control environment plus vehicle; 2) control environment plus esomeprazole (2 mg/kg); 3) stress environment plus vehicle; 4) stress environment plus esomeprazole (2 mg/kg).

研究目的：本研究旨在评估酸抑制对应激小鼠模型大脑转录组的影响，并探讨在存在或不存在应激的情况下，质子泵抑制剂（proton pump inhibitor, PPI）埃索美拉唑对大脑转录组的作用。
研究方法：将雄性C57BL/6小鼠每日置于低温束缚应激环境或对照环境中，持续3天，每日同时以腹腔注射方式给予2 mg/kg埃索美拉唑或生理盐水。采用标准RNA测序（RNA-seq）方法检测小鼠海马体与前额叶皮层的mRNA表达水平。
研究结果：应激与埃索美拉唑均未对前额叶皮层转录组产生显著影响。应激对海马体转录组无明显作用，但联合埃索美拉唑给药可诱导124个基因出现差异表达，其中多数基因参与认知与行为通路。
研究结论：未来需开展进一步研究，以明确微生物群、宿主基因表达、中枢神经系统神经认知调节因子丰度及其对认知与行为的影响之间的关联。
本研究采用Illumina HiSeq 2000（2×151）平台对海马体与前额叶皮层的mRNA进行了转录组谱分析。实验组织样本来源于4组各6只雄性C57BL/6小鼠：1）对照环境+赋形剂组；2）对照环境+埃索美拉唑（2 mg/kg）组；3）应激环境+赋形剂组；4）应激环境+埃索美拉唑（2 mg/kg）组。