Kidney-resident CD8 T cells downregulate IL-18R during maturation

Using the expression of IL-18 receptor (CD218) as a surface marker, CD69+ kidney-resident CD8 T cells can be separated into two subsets. Transcriptional analysis suggests that IL-18R low subset represents the mature tissue-resident T cells 10000 congenically marked naïve P14 T cells were adoptively transferred into each sex-matched unmanipulated C57BL/6 mouse followed by LCMV Armstrong infection via an intraperitoneal route. 12 days after infection, lymphocytes were purified from the kidneys via enzymatic digestion and gradient centrifugation. Indicated P14 T cell subsets were FACS sorted from purified total kidney lymphocytes. Total RNA was extracted from freshly sorted cells. Kidneys from 10-15 recipient mice were pooled for each sorting.

以白细胞介素-18受体（CD218）作为表面标志物，可将CD69阳性肾脏驻留CD8 T细胞分为两个亚群。转录组分析结果表明，IL-18R低表达亚群即为成熟组织驻留T细胞。将10000个经同基因标记的初始P14 T细胞过继转移至每只性别匹配的未处理C57BL/6小鼠体内，随后通过腹腔注射途径感染淋巴细胞脉络丛脑膜炎病毒阿姆斯特丹株（LCMV Armstrong）。感染后12天，通过酶消化与密度梯度离心法从小鼠肾脏中纯化淋巴细胞。从纯化得到的总肾脏淋巴细胞中，通过荧光激活细胞分选术（Fluorescence Activated Cell Sorting, FACS）分选出目标P14 T细胞亚群。从新鲜分选得到的细胞中提取总RNA。每次分选实验需混合10至15只受体小鼠的肾脏组织。