Validation of ten differentially expressed genes with biological replicates using qRT-PCR.

aFold difference for each gene was calculated by FPKM value of PHF-DPCs and SHF-DPCs.bUsing cnpy2 as the internal reference gene to calculate relative gene expression level.cUsing rnf10 as the internal reference gene to calculate relative gene expression level.a. 基于PHF-DPCs与SHF-DPCs的FPKM（Fragments Per Kilobase of transcript per Million mapped reads，每千碱基转录本每百万映射读取片段数）值，计算各基因的差异倍数。 b. 以cnpy2作为内参基因，计算基因相对表达水平。 c. 以rnf10作为内参基因，计算基因相对表达水平。