IFNα-induced gene expression in immortalized pig-tailed macaque CD4+ lymphocytes

In order to identify IFNα-induced host factor(s) that might inhibit replication of unadapted chimeric SIV/HIV-1 viruses (SHIVs), we measured IFNα-induced gene expression in immortalized pig-tailed macaque (Ptm) CD4+ lymphocytes. Triplicate cultures of Ptm lymphocytes were left untreated or treated with 1000 U/ml of IFNα. Twenty-four hours later, RNA was isolated, and RNA-seq libraries were prepared for sequencing. A total of 198 genes were found to be significantly differentially expressed (|logFC| ≥ 0.585 & FDR 5%) upon IFNα treatment. 147/198 genes were found to be significantly upregulated and 51/198 genes were found to be significantly downregulated regulated upon IFNα treatment. IFNα-induced gene expression in immortalized pig-tailed macaque CD4+ lymphocytes left untreated or treated with 1000 U/ml of IFNα.

为鉴定可抑制未适应型嵌合猴免疫缺陷病毒/人类免疫缺陷病毒1型（SHIVs）复制的干扰素α（IFNα）诱导宿主因子，我们对永生化豚尾猴（pig-tailed macaque, Ptm）CD4+淋巴细胞中干扰素α诱导的基因表达进行了检测。将豚尾猴淋巴细胞的三重复孔培养物分为两组，一组不予处理，另一组以1000 U/ml的干扰素α进行处理。培养24小时后，提取总RNA并构建RNA测序（RNA-seq）文库用于测序。经干扰素α处理后，共鉴定出198个显著差异表达基因（|logFC|≥0.585且错误发现率（FDR）≤5%），其中147个基因显著上调，51个基因显著下调。本数据集涵盖未经处理或经1000 U/ml干扰素α处理的永生化豚尾猴CD4+淋巴细胞中的干扰素α诱导基因表达数据。