16S rRNA ONT Nanopore sequencing in water and soil samples

In this study, we evaluated multiple methods including selective enrichment culture, direct PCR, 16S rRNA gene amplification-based sequencing, direct metagenomic sequencing, and Leptospira enrichment culture followed by metagenomic sequencing to detect the presence of Leptospira DNA in environmental samples.本研究评估了多种检测环境样本中钩端螺旋体（Leptospira）DNA的方法，包括选择性富集培养、直接聚合酶链式反应（PCR）、基于16S rRNA基因扩增的测序技术、直接宏基因组测序，以及先进行钩端螺旋体富集培养后开展宏基因组测序的方法。