Expression, Clinical Significance, and Functional Determination of the transmembrane channel-like protein 8 (TMC8) in clear cell renal cell carcinoma

Public data used in the article. A total of 539 ccRCC samples and 72 paracancer samples with basic information (Supplementary Table 1); The DEGs of ccRCC were identified using the screening criteria of an absolute logFC>2, basemean>1000, P<0.0001, and genes biotyped in protein coding (Supplementary Table 2); The LinkedOmics database was utilized to filter for the most relevant genes of TMC8 in ccRCC (Supplementary Table 3); To identify DEGs between TMC8-high and TMC8-low ccRCC patients, DESeq2 was used on TCGA datasets, with genes having an adjusted P value < 0.05 and absolute fold change >1 considered statistically significant (Supplementary Table 4); The number of migratory cells per field was presented in Supplementary Figure S1.

本文所使用的公开数据集包含：共539例肾透明细胞癌（clear cell Renal Cell Carcinoma, ccRCC）样本与72例癌旁样本，均附带基础信息（补充表1）；采用绝对logFC>2、basemean>1000、P<0.0001的筛选标准，且基因类型为蛋白编码基因，鉴定出肾透明细胞癌的差异表达基因（Differentially Expressed Genes, DEGs）（补充表2）；借助LinkedOmics数据库筛选肾透明细胞癌中与TMC8最相关的基因（补充表3）；为鉴定TMC8高表达与低表达肾透明细胞癌患者间的差异表达基因，本研究对癌症基因组图谱（The Cancer Genome Atlas, TCGA）数据集使用DESeq2进行分析，将校正P值<0.05且绝对折叠变化>1的基因视为具有统计学意义（补充表4）；每视野迁移细胞数详见补充图S1。