RNA sequencing of JCPyV infection in primary human astrocytes. RNA sequencing of JCPyV infection in primary human astrocytes

Research in JCPyV infection of primary astrocytes is limited. However, viral infection in a transformed glial cell line (SVGAs), mostly composed of astrocytes, is well studied. RNA sequencing was performed at 24 hours post infection (hpi), 48 hpi, and 96 hpi. Timepoints established to be important in both cell types in the JCPyV infectious cycle. Overall design: Comparitive analysis using RNA sequencing of JCPyV infection during varying timepoints of infection in primary astrocytes versus a transformed glial cell line, mostly composed of astrocytes

针对原代星形胶质细胞的JC多瘤病毒（JCPyV）感染相关研究目前仍较为匮乏。不过，针对主要由星形胶质细胞构成的转化型胶质细胞系SVGAs所开展的病毒感染研究则已有较为充分的研究基础。本研究在感染后24小时（24 hpi）、48小时（48 hpi）及96小时（96 hpi）三个时间点实施了RNA测序（RNA sequencing）；上述三个时间点已被证实为JC多瘤病毒感染周期中两类细胞的关键节点。实验整体设计：通过RNA测序对原代星形胶质细胞与主要由星形胶质细胞构成的转化型胶质细胞系在不同感染时间点的JC多瘤病毒感染情况开展比较分析。