ERK5 sustains autocrine mitogenic balance for cell fate specification in human pluripotent stem cells [MEK4DD RNA-seq]

To investigate the function of MEK5/ERK5 in the regulation of hPSC pluripotency, we processed MAP2K5DD (MEK5DD) overexpression H1 ESC line. We then performed gene expression profiling analysis using data obtained from RNA-seq of MEK5DD-OE H1 ESCs.为探究MEK5/ERK5在人类多能干细胞（human pluripotent stem cell, hPSC）多能性调控中的功能，我们构建了过表达MAP2K5DD（MEK5DD）的H1胚胎干细胞（embryonic stem cell, ESC）系。随后，我们利用MEK5DD过表达（overexpression, OE）的H1胚胎干细胞的RNA测序（RNA-seq）数据开展基因表达谱分析。