Effect of a point mutation at Keap1 C151 on hepatic gene expression in mice treated with Bardoxolone Methyl (CDDO-Me). Effect of a point mutation at Keap1 C151 on hepatic gene expression in mice treated with Bardoxolone Methyl (CDDO-Me)

To investigate the role of Keap1 C151 for induction of gene expression in mouse liver, comprehensive gene expression profiles were compared in the livers of wild-type, Nrf2 knockout, and mice harboring a point mutation at Keap1 C151 (C151S) treated with either CDDO-Me or vehicle Overall design: Gene expression profiling analysis of RNA-seq data in the livers of three genotypes of mice (wild-type, Nrf2-knockout, Keap1 C151S knock-in) treated with one dose (30 umol/kg BW) of CDDO-Me or vehicle after 24 hours

为探究Kelch样ECH结合蛋白1（Keap1）C151位点在小鼠肝脏基因表达诱导中的调控作用，本研究对经CDDO-Me或溶剂处理的野生型、核因子E2相关因子2（Nrf2）敲除型及携带Keap1 C151点突变（C151S）的小鼠肝脏的全面基因表达谱开展了对比分析。
整体实验设计：对三种基因型小鼠（野生型、Nrf2敲除型、Keap1 C151S敲入型）的肝脏RNA测序（RNA-seq）数据进行基因表达谱分析，所有受试小鼠均在接受单剂量（30 μmol/kg 体重）CDDO-Me或溶剂处理24小时后取样。