Supplemental Material for Salim et al., 2021

Figure S1 contains additional validation of the qRIN assay. Figure S2 contains analysis of rDNA stability in fob1D mutants. Figure S3 contains analysis of the loss of MATALPHA-LEU2 in fob1D mutants. Figure S4 contains analysis of rDNA stability in nicotinamide. Figure S5 contains validation of the qRIN assay for the CUP1-MATa reporter strains. Figure S6 contains validation of hits with altered rDNA stability. Figure S7 contains functional validation and additional supporting information with regard to deletion of CUP2 in CUP1-MATa reporter strains. Figure S8 contains stress and locus-specific effects of copper treatment. Figure S9 contains data regarding how deletion of RTT109, HST3, and HST4 affect stability of TUB1. Figure S10 contains data regarding how H3K56 acetylation restricts transcription-induced amplification of the CUP1 array in a 17 copy strain. File S1 describes how to calculate repeat loss rates. File S2 describes the method for determining and the location of the MATa-LEU2 casette in reporter strains. Table S1 summarizes rDNA and CUP1 instability measurements from the literature. Table S2 is a list of yeast strains. Table S3 is a list of primers. Table S4 contains validation copy number measurements for 25S, CUP1, LEU2 and MATALPHA1 in various yeast strains. Table S5 contains the rDNA repeat loss rates and copy number measurements from the screens. Table S6 contains rDNA copy number measurements from the temperature sensitive mutant collection. Table S7 contains copy number measurements of subcultured CUP1-MATa strains.补充图S1包含qRIN检测（qRIN assay）的额外验证实验数据。 补充图S2包含fob1Δ突变体中核糖体DNA（rDNA）稳定性的分析数据。 补充图S3包含fob1Δ突变体中MATALPHA-LEU2位点丢失情况的分析数据。 补充图S4包含烟酰胺（nicotinamide）处理下核糖体DNA稳定性的分析数据。 补充图S5包含针对CUP1-MATa报告菌株（reporter strains）的qRIN检测验证实验。 补充图S6包含对核糖体DNA稳定性发生改变的命中靶点的验证实验。 补充图S7包含CUP1-MATa报告菌株中CUP2基因缺失的功能验证实验及额外辅助支撑数据。 补充图S8包含铜离子处理的应激效应及位点特异性效应分析。 补充图S9包含RTT109、HST3与HST4基因缺失对TUB1基因稳定性影响的相关数据。 补充图S10包含组蛋白H3K56乙酰化（H3K56 acetylation）如何限制17拷贝菌株中CUP1阵列转录诱导扩增的相关数据。 文件S1描述了重复序列丢失率的计算方法。 文件S2描述了报告菌株中MATa-LEU2基因盒（casette）的定位方法及其整合位置。 表格S1汇总了已发表文献中核糖体DNA与CUP1不稳定性的检测数据。 表格S2为酵母菌株列表。 表格S3为引物序列列表。 表格S4包含不同酵母菌株中25S、CUP1、LEU2及MATALPHA1的验证性拷贝数检测数据。 表格S5包含筛选实验获得的核糖体DNA重复序列丢失率及拷贝数检测数据。 表格S6包含温度敏感突变体集合的核糖体DNA拷贝数检测数据。 表格S7包含连续传代培养的CUP1-MATa菌株的拷贝数检测数据。