RNAseq of chicken macrophages from MDV susceptible and resistant chicken lines

Chicken bone marrow-derived macrophages (BMDMs) from lines 6(1) and 7(2) were infected with MDV in vitro. Upon infection, control and infected BMDMs were sorted on 1 dpi. Three separate biological replicates for each of the control and infected BMDMs were used for RNA preparation and subsequent sequencing. Chakraborty P, Kuo R, Vervelde L, Dutia BM, Kaiser P, Smith J. Macrophages from Susceptible and Resistant Chicken Lines have Different Transcriptomes following Marek's Disease Virus Infection. Genes (Basel). 2019 Jan 22;10(2). pii: E74. doi: 10.3390/genes10020074. PubMed PMID: 30678299.

本研究以品系6(1)与品系7(2)的鸡骨髓源巨噬细胞（bone marrow-derived macrophages, BMDMs）为实验材料，体外感染马立克氏病病毒（Marek's Disease Virus, MDV）。感染后，于感染后第1天（1 dpi）对对照组与感染组的BMDMs进行分选。对照组与感染组的BMDMs各设置3个独立生物学重复，用于RNA提取及后续测序。Chakraborty P、Kuo R、Vervelde L、Dutia BM、Kaiser P、Smith J. 易感与抗性鸡品系来源的巨噬细胞在马立克氏病病毒感染后转录组存在差异. 《Genes (Basel)》. 2019年1月22日;10(2). 文章编号：E74. DOI：10.3390/genes10020074. PubMed PMID：30678299.