The effects of X-ray radiation on pancreatic cancer cells

Dying pancreatic cancer cells from chemoradiation can directly stimulate repopulation of surviving cells. We used microarrays to conduct the analysis of whole transcript profiles in pancreatic cancer cells treated with X-ray radiation. Pancreatic cancer cells were seeded into culture plate overnight with 2% FBS in culture medium and then were irradiated with 10Gy using an Oncor linear accelerator. Irradiated pancreatic cancer cells were collected at 24h after X-ray radiation for RNA extraction and hybridization on Affymetrix microarray. We sought to obtain whole transcript profiles in pancreatic cancer cells treated with X-ray radiation.

经放化疗后濒死的胰腺癌细胞可直接刺激存活肿瘤细胞的再增殖。我们采用微阵列（microarrays）技术，对经X射线辐射处理的胰腺癌细胞的全转录组谱展开分析。将胰腺癌细胞以含2%胎牛血清（Fetal Bovine Serum, FBS）的培养液接种于培养板中过夜培养，随后使用Oncor直线加速器以10Gy剂量进行X射线辐射。于X射线辐射后24小时收集受辐射的胰腺癌细胞，进行RNA提取并在Affymetrix微阵列上完成杂交反应。本研究旨在获取经X射线辐射处理的胰腺癌细胞的全转录组表达谱。