Nuclear functions of KaeA, the subunit of KEOPS complex in Aspergillus nidulans.

The aim of this project is to confirm that KaeA a subunit of highly conserved KEOPS/EKC complex, participates in the transcription regulation in Aspergillus nidulans. To identify genes directly or indirectly regulated by KaeA, the RNA-seq experiments were performed using kaeA mutants and the wild type kaeA+ strain. Overall design: Comparative gene expression profiling analysis of RNA-seq data for kaeA19 and kaeA25 mutants and their isogenic wild-type strains, grown in minimal medium in three biological replicates.

本研究旨在证实：作为高度保守的KEOPS/EKC复合物亚基的KaeA，参与构巢曲霉（Aspergillus nidulans）的转录调控过程。为鉴定受KaeA直接或间接调控的基因，本研究利用kaeA突变体与野生型kaeA+菌株开展了RNA测序（RNA-seq）实验。实验总体设计如下：对在基础培养基中培养的kaeA19、kaeA25突变体及其同基因背景野生型菌株的RNA-seq数据进行比较基因表达谱分析，实验设置3次生物学重复。