Dominant yeasts associated to mango (Mangifera indica) and rose apple (Syzygium malaccense) fruit pulps investigated by culture-based methods

Abstract: The biotechnological potential of yeasts associated to different habitats in Colombia has been poorly studied, especially the yeasts associated with different plant structures. Fruit pulps are interesting substrates mainly for the growth of yeast species, that can positively affect the productivity and quality of some bioeconomic species. Therefore, the objective of this study was to identify the dominant yeast species associated with mango and rose apple fruit pulps in Cali, Colombia. A total of 90 isolates were obtained, which were grouped considering their colony morphology. The D1/D2 domain of the large ribosomal RNA gene (LSU rRNA gene) or internal transcribed spacer (ITS) 1, ribosomal gene 5.8S and ITS 2 (ITS) regions of one to several representative isolates from each group was sequenced and compared with type strains for identification. The species Hanseniaspora thailandica, H. opuntiae and Clavispora lusitaniae were reported as shared by both fruits, specific for rose apple (H. uvarum, Pichia terricola, Rhodosporidiobolus ruineniae and Candida albicans), or for Mango (Meyerozyma caribbica, M. guilliermondii, C. natalensis, Aureobasidium pullulans, Pichia sp., Saturnispora diversa and C. jaroonii). Two morphotypes were not identified at the taxonomic level of species and were reported as candidates for new species, belonging to the genera Wickerhamomyces and Pichia.

摘要：栖息于哥伦比亚不同生境的酵母菌的生物技术潜力尚未得到充分研究，其中与不同植物结构相关的酵母菌类群更是鲜有报道。果肉是酵母菌生长的优质基质，可对部分生物经济物种的产量与品质产生积极影响。为此，本研究旨在对哥伦比亚卡利市芒果和蒲桃（rose apple）果肉中的优势酵母菌类群开展鉴定。本研究共获得90株酵母菌分离物，并依据菌落形态对其进行分组。针对每组中的1至多株代表性分离物，对核糖体大亚基RNA基因（large ribosomal RNA gene, LSU rRNA gene）的D1/D2结构域，或核糖体5.8S基因及内部转录间隔区（internal transcribed spacer, ITS）的ITS1、ITS2区域进行测序，并与模式菌株序列比对以完成物种鉴定。经鉴定，泰国汉逊酵母（Hanseniaspora thailandica）、仙人掌汉逊酵母（H. opuntiae）以及卢西尼亚棒孢酵母（Clavispora lusitaniae）为两种水果果肉共有的酵母菌类群；另有若干酵母菌仅在蒲桃果肉中检出，包括汉逊酵母（H. uvarum）、土壤毕赤酵母（Pichia terricola）、鲁伊纳红冬孢酵母（Rhodosporidiobolus ruineniae）以及白色假丝酵母（Candida albicans）；还有部分酵母菌仅在芒果果肉中检出，包括加勒比梅奇酵母（Meyerozyma caribbica）、季也蒙梅奇酵母（M. guilliermondii）、纳塔尔假丝酵母（C. natalensis）、出芽短梗霉（Aureobasidium pullulans）、毕赤酵母属未定种（Pichia sp.）、多样土星酵母（Saturnispora diversa）以及贾鲁恩假丝酵母（C. jaroonii）。另有2种形态型未能鉴定至物种水平，分别归属于威克汉姆酵母属（Wickerhamomyces）和毕赤酵母属（Pichia），为潜在新物种候选菌株。