Quantification of endosomes in Fig 7.

Relative numbers of endosomes (Rab5, Rab7, Rab11, btl>Lamp-GFP) were analyzed in late-stage 16 wild-type control, Osi9+15+19 mutants, and btl>Osi9 embryos. The relative numbers of endosomes were normalized against the average numbers in control, which was set as 1. The relative numbers of Lamp in control and Osi mutants were shown in the pink area. The relative numbers of Rab7 in Osi mutants, wild type control, and btl>Osi9 embryos were shown in yellow area. The relative numbers of Rab11 in Osi mutants, wild type control, and btl>Osi9 embryos were shown in green area. The relative number of Rab5 in control and Osi mutants were shown in blue area.
(XLSX)

本研究对16期晚期野生型对照、Osi9+15+19突变体以及btl>Osi9胚胎中的内体（endosomes）相对数量进行了分析，所检测的内体标记物涵盖Rab5、Rab7、Rab11以及btl>Lamp-GFP。实验以野生型对照样本的内体平均数量为基准（设为1），对其余各组样本的内体相对数量完成归一化处理。其中，对照组与Osi突变体的Lamp相对数量以粉色区域呈现；Osi突变体、野生型对照及btl>Osi9胚胎的Rab7相对数量以黄色区域呈现；Osi突变体、野生型对照及btl>Osi9胚胎的Rab11相对数量以绿色区域呈现；对照组与Osi突变体的Rab5相对数量以蓝色区域呈现。
(XLSX)