Genomics and Proteomics Provide New Insight into the Commensal and Pathogenic Lifestyles of Bovine and Human-associated Staphylococcus epidermidis

The goal of the present study is to understand mechanisms by which SE can infect the bovine host and persist in the mammary gland. For this purpose we determined the whole genome and four plasmid sequences of the SE strain PM221 originating from spontaneous persistent bovine IMI. We compared the genomic data with those of ATCC12228 and RP62A to screen for conserved and acquired gene pools. To confirm and complement these findings two-dimensional difference gel electrophoresis (2D DIGE) involving each three strains, surfome shaving and experimental infections in a bovine model was applied to indicate potential differences.

本研究旨在阐明SE感染牛宿主并在乳腺中持续定植的分子机制。为此，我们对源自自发性持续性牛乳腺内感染（Intramammary Infection，IMI）的SE菌株PM221的全基因组及4条质粒序列进行了测定。我们将该基因组数据与ATCC12228和RP62A的基因组数据进行比对，以筛选保守基因库与获得性基因库。为验证并补充上述研究结果，我们采用了涉及3株受试菌株的双向差异凝胶电泳（two-dimensional difference gel electrophoresis，2D DIGE）、表面蛋白质组刮削技术（surfome shaving）以及牛模型感染实验，以揭示潜在的差异特征。