Figure 1 TPL R6

We do site-saturation mutagenesis to look at the repressive function of residueR6 in H1 of LisH domain in TPL. We want to understand more about the role of this residue in inducible de-repressoin of auxin responsive transcription.
Conclusion:
we find most mutations here do not interfere with repressive ability. We have a negative control (no repression alpha helix control, and no repression noIAA3 and no H1 control) and a positive control (strong repressor TPL H1). We gate for live haploid yeast cells. We dilute in the morning and wait to log growth to make fluorescence measurements.

本研究采用定点饱和诱变（site-saturation mutagenesis）技术，探究TPL中LisH结构域（LisH domain）H1区段内残基R6的阻遏功能，旨在进一步阐明该残基在生长素响应转录的诱导去阻遏过程中所发挥的作用。
结论：
本研究发现，绝大多数诱变突变不会对其阻遏能力造成影响。实验设置了阴性对照（无阻遏α螺旋对照、无阻遏noIAA3对照以及无H1对照）与阳性对照（强效阻遏因子TPL H1）。过程中我们通过门控策略筛选活的单倍体酵母细胞，并于清晨进行细胞稀释，待其生长至对数生长期后开展荧光检测。