Effective seed sterilization methods require optimization across maize genotypes

# Effective seed sterilization methods require optimization across maize genotypes [https://doi.org/10.5061/dryad.bcc2fqzmb](https://doi.org/10.5061/dryad.bcc2fqzmb) Excel files 'Experiment 1' and 'Experiment 2' contain raw data. R markdown file 'Seed_Sterlization_Manuscript' contains R code used for data analysis. Text files 'B73' and 'SugarBun' were used in the R script. #### Descriptions of data in Excel files 'Experiment 1' and 'Experiment 2': * The genotypes used in the study were ((all were used in experiment 2, only B73 and Sugar Bun were used in experiment 1): 1. Inbred varieties: B73, Mo17 2. Modern (hybrid) varieties: Sugar Bun (SB), Mo17 x B73, B73 x Mo17 * Several sources of seed were used for experiment 2. Each case would have a source as well as a genotype used (see above): 1. Clayton field seed harvested in 2021: CL21 2. Clayton field seed harvested in 2022: CL22 3. Seeds harvested from a greenhouse sown in 2021, harvested in 2022: GH21/22 4. Seeds harvested from Mexico fields (sown in 2021): MX21/22 * Germination and contamination were recorded for each seed in a 24-well plate. Positive for germination is recorded as "1", contamination is recorded as "1". The letters (A-D) and numbers (1-6) represent the coordinates for each seed in the plate. * For experiment 1, two seed sterilization protocols were tested, a 'Long' and a 'Short' protocol. Variations in each protocol included a 'Pre Soak' step, and the germination of sterilized seeds on 'Fungicide'.

# 有效种子灭菌方法需针对玉米基因型进行优化 [https://doi.org/10.5061/dryad.bcc2fqzmb](https://doi.org/10.5061/dryad.bcc2fqzmb)
Excel文件"Experiment 1"与"Experiment 2"包含原始实验数据。R Markdown文件"Seed_Sterlization_Manuscript"包含用于数据分析的R代码。文本文件"B73"与"SugarBun"为R脚本中所调用的资源。
#### Excel文件"Experiment 1"与"Experiment 2"的数据说明：
* 本研究使用的基因型（实验2涵盖全部基因型，实验1仅使用B73与Sugar Bun）分为两类：
1. 自交系品种：B73、Mo17
2. 现代（杂交）品种：Sugar Bun（SB）、Mo17×B73、B73×Mo17
* 实验2使用了多批次种子，每一批次均对应特定来源与基因型（详见上文）：
1. 2021年收获于Clayton试验田的种子：标记为CL21
2. 2022年收获于Clayton试验田的种子：标记为CL22
3. 2021年播种于温室、2022年收获的种子：标记为GH21/22
4. 2021年播种于墨西哥试验田并收获的种子：标记为MX21/22
* 针对24孔板中的每粒种子，记录其萌发与污染情况：发芽阳性记为"1"，污染阳性亦记为"1"；字母（A-D）与数字（1-6）代表该孔在板内的坐标位置。
* 实验1共测试了两种种子灭菌方案，分别为"长时"与"短时"灭菌方案；两种方案均包含"预浸泡"步骤，且灭菌后的种子均在含杀菌剂的培养基上开展萌发实验。