Research on the mode of action of a novel 2-cyclopropyl-4-aminopyrimidine hydrazine compound (4k) for control of cyanobacteria. Research on the mode of action of a novel 2-cyclopropyl-4-aminopyrimidine hydrazine compound (4k) for control of cyanobacteria

We reported the transcriptomics files of cyanobacteria treated by compound 4k, which exhibited a good algicidal activity. The results revealed that the transcription of genes in the photosynthesis pathways, such as thylakoid and PSI pathway, were affected by 4k, which was validated by the qPCR results. Photosynthesis parameters was further measured and showed that photosynthesis were significantly affected by 4k treatments. Overall design: mRNA profiles of Synechocystis sp. PCC6803 cells treated by 1 μM and 3 μM of 4k, using DMSO treatment as a control. Three independent biological replicates were performed for each treatment.

本研究报道了经化合物4k处理后的蓝藻转录组学数据集，该化合物具备优异的杀藻活性。研究结果显示，类囊体(thylakoid)、光系统I(PSI)等光合通路的基因转录水平均受到4k的调控，该结论经实时荧光定量PCR(qPCR)验证。本研究进一步测定了光合相关参数，结果证实4k处理可显著影响蓝藻的光合生理功能。总体实验设计：以二甲基亚砜(DMSO)处理组作为对照，对集胞藻(Synechocystis sp.) PCC6803细胞施加1 μM与3 μM浓度的4k进行处理；每组处理均设置三次独立生物学重复。