Single worm DNAseq for hybrid male inviability

Wild-type C. briggsae and C. nigoni can produce fertile hybrid XX female progeny, but XO progeny are either 100% inviable (when C. briggsae is the mother) or viable but sterile (when C. nigoni is the mother). As F1 male progeny of C. briggsae (father) X C. nigoni ZZY10337 (mother) crosses are fertile, we can use these F1 males to screen for specific ChrX-autosome interactions that might contribute to hybrid male inviability. To this end, we crossed fertile F1 males with C. briggsae hermaphrodites, then collected the resulting viable B2 males for genotyping by single-worm genome sequencing

野生型C. briggsae与C. nigoni可产生可育的XX型雌性杂交后代，但其XO型后代的存活情况存在差异：当母本为C. briggsae时，XO后代100%不可存活；当母本为C. nigoni时，XO后代虽可存活但不育。由于以C. briggsae为父本、C. nigoni ZZY10337为母本的杂交组合所产生的F1雄性后代可育，我们可借助这些F1雄性个体，筛选可能参与介导杂交雄性致死的特定X染色体-常染色体互作关系。为此，我们将可育的F1雄性与C. briggsae雌雄同体个体进行杂交，随后收集获得的可存活B2雄性个体，通过单线虫基因组测序完成基因分型。