Overall comparison of putative reference genes’ stability.

Results are calculated for all samples for each donor, i.e. statin-treated cells and combined statin-and-TNF-α-treated cells. Rankings are based on geNorm stability M-values, NormFinder stability values and coefficient of correlation values (r) counted by BestKeeper. SD values calculated by BestKeeper are also given in the table. The genes with SD >1 are eliminated from further analysis. For the overall final ranking the geometric mean of the weights (GeoMean) assigned by the rankings from all three programs was calculated.本研究针对每位供体的所有样本计算结果，即仅经他汀类药物（statin）处理的细胞，以及经他汀类药物联合肿瘤坏死因子α（TNF-α）处理的细胞。基因排名依据geNorm稳定性M值、NormFinder稳定性值，以及由BestKeeper统计得到的相关系数（r）确定。表格中同时列出了BestKeeper计算得到的标准差（Standard Deviation, SD）值。将标准差大于1的基因剔除，不纳入后续分析。最终综合排名通过计算三款分析程序各自给出的排名所对应权重的几何均值（Geometric Mean, GeoMean）得到。