CIL:36995, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

Specifications of the custom built two-photon microscope, including the laser sources, signal amplification, PMT specifications, and YFP filter sets are described in:
Ruthazer ES, Li J, Cline HT. 2006. Stabilization of axon branch dynamics by synaptic maturation. J Neurosci 26(13):3594-3603.

The methods of cell transfection with electroporation can be found here:
Bestman JE, Ewald RC, Chiu S-L, Cline HT. 2006. In vivo single-cell electroporation for transfer of DNA and macromolecules. Nat Protocols 1(3):1267-1272.

Further explanation can be found here:
Bestman JE, Cline HT. 2008. The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proc Natl Acad Sci U S A 105(51):20494-20499.

Bestman JE, Cline HT. 2009. The Relationship between Dendritic Branch Dynamics and CPEB-Labeled RNP Granules Captured in Vivo. Front Neural Circuits 3:10.

本定制双光子显微镜的各项技术规格，包括激光光源、信号放大系统、光电倍增管(PMT)规格以及黄色荧光蛋白(YFP)滤光片组配置，详见以下文献：
Ruthazer ES、Li J、Cline HT. 2006年. 突触成熟对轴突分支动态的稳定作用. 《神经科学杂志》(J Neurosci) 26(13):3594-3603.

细胞电穿孔转染方法详见以下文献：
Bestman JE、Ewald RC、Chiu S-L、Cline HT. 2006年. 用于DNA与大分子转染的活体细胞单电穿孔技术. 《自然实验手册》(Nat Protocols) 1(3):1267-1272.

更多相关说明详见以下文献：
Bestman JE、Cline HT. 2008年. RNA结合蛋白CPEB在活体内调控树突形态发生与神经元环路组装. 《美国国家科学院院刊》(Proc Natl Acad Sci U S A) 105(51):20494-20499.
Bestman JE、Cline HT. 2009年. 活体内捕获的树突分支动态与CPEB标记的核糖核蛋白(RNP)颗粒之间的关联. 《前沿神经环路》(Front Neural Circuits) 3:10.