H3K9me3 (new lot).D.mel 3rd Instar Larvae Nuclei Sexed Male Jil mutant

modENCODE_submission_5623 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Sexed male Jil mutant(official name : Jil-1 z2 genotype : Jil-1 z2/Jil-1z2 outcross : 1 transgene : deletion tags : Transposon tag : none description : - The JIL-1 allele JIL-1z2 was isolated in a screen for imprecise excisions of the EP transposon (Rørth et al. description : 1998) in w; EP(3)3657/?2?3 Sb heterozygotes Developmental Stage: 3rd Instar Larvae Sexed Male; Genotype: Jil-1 z2/Jil-1z2; Sex: Male; Transgene: deletion; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain Sexed male Jil mutant(official name : Jil-1 z2 genotype : Jil-1 z2/Jil-1z2 outcross : 1 transgene : deletion tags : Transposon tag : none description : - The JIL-1 allele JIL-1z2 was isolated in a screen for imprecise excisions of the EP transposon (Rørth et al. description : 1998) in w; EP(3)3657/?2?3 Sb heterozygotes Antibody H3K9me3 (new lot) (target is H3K9me3); Developmental Stage 3rd Instar Larvae Sexed Male

modENCODE_submission_5623 本提交项隶属于Gary Karpen主持的modENCODE（模式生物基因组元件百科全书）项目。完整modENCODE项目列表请参见：http://www.genome.gov/26524648。
项目目标：本研究旨在定位黑腹果蝇（Drosophila melanogaster）基因组中主要的组蛋白修饰位点。所研究的组蛋白修饰参与DNA复制、基因表达、基因沉默及遗传等核心染色体生物学功能。本研究将采用染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）结合基因组平铺芯片的实验策略。
研究初期将使用3种细胞系与2个胚胎阶段的染色质开展定位分析，后续将把部分蛋白的分析拓展至另外4种动物组织/发育阶段。
数据使用条款与条件请参阅：http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
实验类型：CHIP-chip。
生物样本来源：
品系：性别分化的雄性Jil突变体（官方命名：Jil-1 z2；基因型：Jil-1 z2/Jil-1z2；远交次数：1；转基因情况：缺失；标签：转座子标签：无；描述：- Jil-1等位基因Jil-1z2是通过对w; EP(3)3657/?2?3 Sb杂合子中EP转座子的非精确切离进行筛选分离得到的（Rørth等人，1998））
发育阶段：3龄幼虫 性别分化的雄性；基因型：Jil-1 z2/Jil-1z2；性别：雄性；转基因情况：缺失；
重复次数：4；
实验因素：
品系：性别分化的雄性Jil突变体（官方命名：Jil-1 z2；基因型：Jil-1 z2/Jil-1z2；远交次数：1；转基因情况：缺失；标签：转座子标签：无；描述：- Jil-1等位基因Jil-1z2是通过对w; EP(3)3657/?2?3 Sb杂合子中EP转座子的非精确切离进行筛选分离得到的（Rørth等人，1998））
抗体：H3K9me3（新批次，靶标为H3K9me3）；发育阶段：3龄幼虫 性别分化的雄性