Molecular insight into the Lin28R192G mutation in human ESCs. Molecular insight into the Lin28R192G mutation in human ESCs

Total RNA-seq analyses for the differentially expressed genes (DEGs) among WT (c42), Lin28 R192G hetero mut hESC (c26_5), and Lin28 R192G homo mut hESC (c38) To gain further molecular insight into the observed Lin28R192G mutation in human ESCs, we performed total RNA-sequencing (RNA-seq) analysis of the WT, Lin28 R192G hetero mut hESC, and Lin28 R192G homo mut hESC. The genes that are differentially expressed (DEGs) in Lin28 R192G hetero mut and Lin28 R192G homo mut hES compared to WT hESC. Overall design: 3 human samples: WT hESC (c42), Lin28 R192G hetero mut hESC (c26_5), and Lin28 R192G homo mut hESC (c38)

针对野生型（WT）、Lin28 R192G杂合突变人类胚胎干细胞（human embryonic stem cell, hESC，c26_5）及Lin28 R192G纯合突变人类胚胎干细胞（human embryonic stem cell, hESC，c38）三组样本的差异表达基因（Differentially Expressed Genes，DEGs）开展总RNA测序分析。为深入解析人类胚胎干细胞中Lin28 R192G突变的分子机制，我们对上述三组样本进行了总RNA测序（RNA-seq）分析，旨在获取相较于野生型人类胚胎干细胞，Lin28 R192G杂合突变与纯合突变人类胚胎干细胞中的差异表达基因（DEGs）。实验整体设计：共包含3组人类样本，分别为野生型人类胚胎干细胞（WT hESC，c42）、Lin28 R192G杂合突变人类胚胎干细胞（c26_5）及Lin28 R192G纯合突变人类胚胎干细胞（c38）。