Study of the influence of the presence of Dr fimbriae on the adherence of cells of the recombinant Escherichia coli strain: AAEC191A/pCC90 to polystyrene in a dynamic system - PBS pH=5,7

The attachment of bacteria begins the process of surface colonization, called biofilm development, characterized by a number of physicochemical and molecular interactions. Adherence to inert surfaces typically involves nonspecific interactions, whereas adherence to biological surfaces is associated with specific ligand-receptor interactions. In this study, the adherence ability of two genetically modified Escherichia coli strains— AAEC191A/pCC90, and AAEC191A/pACYCpBAD—was examined on both glass and polystyrene surfaces. The aim was to investigate the effect of the presence of Dr fimbriae on the adherence of bacteria to various carriers. The experiments were performed under various flow conditions. cells were suspended in phosphate buffers of different pH=5.7, pH=7 and pH=8
The catalog contains original video data that can be played using Olympus' CellSense software. The videos contain full layers of data characterizing the image recording process.

细菌附着启动了表面定殖过程，该过程又称生物膜形成（biofilm development），以一系列物理化学与分子相互作用为特征。细菌对惰性表面的黏附通常涉及非特异性相互作用，而对生物表面的黏附则与特异性配体-受体相互作用相关。本研究针对两种基因工程改造的大肠埃希菌（Escherichia coli）菌株——AAEC191A/pCC90与AAEC191A/pACYCpBAD——在玻璃及聚苯乙烯表面的黏附能力展开了检测，旨在探究Dr菌毛（Dr fimbriae）的存在对细菌黏附各类载体的影响。实验于多种流体条件下开展，菌体被悬浮于pH值分别为5.7、7.0及8.0的磷酸盐缓冲液中。
本数据集目录包含原始视频数据，可通过奥林巴斯（Olympus）的CellSense软件进行播放。这些视频包含了表征图像记录过程的全层级数据。